TY - JOUR
T1 - Evaluación de las pruebas de PCR TcH2AF-R y S35-S36 para la detección de Trypanosoma cruzi en tejido cardiaco de ratón
AU - Barrera, Yuli Katherine
AU - Guevara, Johana María
AU - Pavía, Paula Ximena
AU - Montilla, Marleny
AU - Nicholls, Rubén Santiago
AU - Parra, Edgar
AU - Puerta, Concepción Judith
PY - 2008
Y1 - 2008
N2 - Introduction: Heart transplant is a therapeutic option in the treatment of chagasic cardiomyopathy. For early detection of Chagas reactivation cases, the use of PCR tests using endomyocardial biopsies has been proposed. Development of an animal model will be the first step in evaluating the applicability of this approach. Objective: PCR tests based on the TcH2AF-R and S35-S36 primers were evaluated for the detection of T. cruzi in heart tissue of mice experimentally infected with the parasite. Materials and methods: Two groups of ICR mice of 15 and 10 individuals were infected by intraperitoneal injection with 0.3 ml of PBS containing 1×106 trypomastigotes of the MHOM/CO/2001/D.A. (T. cruzi I) strain or 1×104 trypomastigotes of MHOM/BR/00/Y (T. cruzi II) strain. Parasitemia and cardiac parasitic infection were determined at 30, 60 (acute model), 100 and 150 (chronic model) days by means of histopathological examination and by PCR, using the TcH2AF-R and S35-S36 primers. Results: The histopathological findings revealed alterations in the heart and the presence of intracellular amastigotes in acute and chronic models. In contrast to parasitemia levels and histopathological analyses, S35-S36 PCR detected infections in mice that were infected with either parasite strain. TcH2AF-R PCR detected T. cruzi I-infected mice earlier and more frequently than inspection for parasitemia or histopathological examination. Conclusions: Applying PCR tests with both primers proved superior for Chagas disease confirmation over currently standard detection methods.
AB - Introduction: Heart transplant is a therapeutic option in the treatment of chagasic cardiomyopathy. For early detection of Chagas reactivation cases, the use of PCR tests using endomyocardial biopsies has been proposed. Development of an animal model will be the first step in evaluating the applicability of this approach. Objective: PCR tests based on the TcH2AF-R and S35-S36 primers were evaluated for the detection of T. cruzi in heart tissue of mice experimentally infected with the parasite. Materials and methods: Two groups of ICR mice of 15 and 10 individuals were infected by intraperitoneal injection with 0.3 ml of PBS containing 1×106 trypomastigotes of the MHOM/CO/2001/D.A. (T. cruzi I) strain or 1×104 trypomastigotes of MHOM/BR/00/Y (T. cruzi II) strain. Parasitemia and cardiac parasitic infection were determined at 30, 60 (acute model), 100 and 150 (chronic model) days by means of histopathological examination and by PCR, using the TcH2AF-R and S35-S36 primers. Results: The histopathological findings revealed alterations in the heart and the presence of intracellular amastigotes in acute and chronic models. In contrast to parasitemia levels and histopathological analyses, S35-S36 PCR detected infections in mice that were infected with either parasite strain. TcH2AF-R PCR detected T. cruzi I-infected mice earlier and more frequently than inspection for parasitemia or histopathological examination. Conclusions: Applying PCR tests with both primers proved superior for Chagas disease confirmation over currently standard detection methods.
KW - Chagas cardiomyopathy
KW - Chagas disease
KW - Polymerase chain reaction
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=66549112415&partnerID=8YFLogxK
U2 - 10.7705/biomedica.v28i4.68
DO - 10.7705/biomedica.v28i4.68
M3 - Artículo
C2 - 19462567
AN - SCOPUS:66549112415
SN - 0120-4157
VL - 28
SP - 616
EP - 626
JO - Biomedica
JF - Biomedica
IS - 4
ER -
