Comparación de una prueba de PCR basada en los genes codificantes para histora HSA/SIRE con pruebas serológicas convencionales para el diagnóstic de la enfermedad de Chagas crónica eb pacientes Colombianos

Juliana Gil, Paula Pavía, Marleny Montilla, Astrid C. Florez, Claudia Quintero, Marcela Mercado, Miguel Vacca, Santiago Nicholls, Concepción Puerta

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

15 Citas (Scopus)

Resumen

Introduction. Diagnosis of Chagas disease in its latent and chronic phase is difficult because of the low parasiternia levels. Therefore, serological and molecular techniques are necessary to achieve an appropriate diagnosis. Objective. The polymerase chain reaction based on the amplification of the SIRE element inserted into H2A encoding genes was compared with classical serological tests for the diagnosis of Chagas disease in Colombian patients. Materials and methods. An agreement study was carried out by comparing the PCR with ELISA (enzyme linked immuno sorbent assay) and IFAT (indirect immunofluorescence) tests. In addition, the PCR sensitivity and specificity were determined. A sample of 156 individuals was tested with the H2A PCR primers after a Chagas disease classification based on clinical, epidemiological and serological data associated with each patient. In addition, 97 out of 156 samples were also compared with the S35/S36 PCR primers. Results. Eighty-nine of 156 samples (57%) were positive by both IFAT and ELISA and 84 (53.8%) presented the expected 234 bp amplification fragment. The sensitivity of the TcH2AF/R PCR was 88% (95% C.I.: 75% - 95%) and its specificity 92.5% (95% C.I.: 87.7% - 97.2%). The kappa index for concordance between serological tests and TcH2AF/R PCR was 0.8 (95% C.I.: 73% - 86%), and between the TcH2AF/R and S35/S36 PCR primers was 0.9 (95% C.I.: 84% - 96%). These indices indicated a "good" and "almost perfect" agreement, respectively. Conclusions. The TcH2AF/R PCR is a promising diagnostic tool for the detection of T. cruzi and is suggested as a tool complementary to the classical serological tests.

Título traducido de la contribuciónComparison of PCR test based on the histone H2A/SIRE genes with classical serological tests for the diagnosis of chronic chagas disease in Colombian patients
Idioma originalEspañol
Páginas (desde-hasta)83-91
Número de páginas9
PublicaciónBiomedica
Volumen27
N.ºSUPPL. 1
DOI
EstadoPublicada - ene. 2007
Publicado de forma externa

Palabras clave

  • Chagas myocardiopathy
  • Histories
  • Polymerase chain reaction
  • Trypanosoma cruzi

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